Authors: Marques, LLR; Oosterbroek, L; Caro-Riano, H; Ehman, C; Zwierink, M


DOI https://doi.org/10.36487/ACG_rep/1152_32_Zwierink

Cite As:
Marques, LLR, Oosterbroek, L, Caro-Riano, H, Ehman, C & Zwierink, M 2011, 'Detection of soil sulphur oxidising bacteria on Suncor’s reclamation material stockpiles and the reclaimed landscape', in AB Fourie, M Tibbett & A Beersing (eds), Proceedings of the Sixth International Conference on Mine Closure, Australian Centre for Geomechanics, Perth, pp. 291-299, https://doi.org/10.36487/ACG_rep/1152_32_Zwierink

Download citation as:   ris   bibtex   endnote   text   Zotero


Abstract:
Suncor Energy Inc. (Suncor) is currently reclaiming some areas to jackpine a1 and b1 ecosites. These ecotypes require slightly acidic to acidic soils. However, much of the existing soil on the reclaimed landscape and in many of the reclamation stockpiles has a neutral to slightly basic soil reaction. Tests were carried out on reclamation peat/mineral mix stockpiles and on the reclaimed landscape to determine the presence of soil sulphur oxidising bacteria (SOB). These bacteria can be used with additions of elemental sulphur to acidify the neutral to basic reclamation material and provide a substrate for the establishment and development of jackpine vegetative communities. HydroQual Laboratories Ltd. (HydroQual), Golder Associates Ltd. (Golder) and Suncor carried out a study to determine the presence of two SOB bacteria species in 90 soil samples (180 DNA tests in total). The two SOB species were chosen based on previous studies described in the scientific literature. Sixty composite soil samples at ten reclamation peat/mineral mix reclamation material stockpiles were sampled at 0 to 20 cm and 1+ m depths. Thirty composite soils samples were collected from ten reclaimed landscape sites. Three replicate composite samples were collected at each site. Testing was carried out to determine presence/absence of SOB in soil samples through standard DNA detection of SOB by polymerase chain reaction (PCR). Soil samples were also analysed for basic soil parameters such as pH, electrical conductivity (EC), sodium adsorption ratio (SAR), cation exchange capacity (CEC), total nitrogen, organic carbon and particle size analysis. The sampling program allowed for the statistical comparison of soil parameters using Analysis of Variance (ANOVA).

References:
Baar, C., d’Abbadie, M., Vaisman, A., Arana, M.E., Hofreiter, M., Woodgate, R., Kunkel, T.A. and Holliger, P. (2011) Molecular breeding of polymerases for resistance to environmental inhibitors, Nucleic Acid Research, pp. 1–12, .
Barbosa, V.L., Atkins, S.D., Barbosa, V.P., Burgess, J.E. and Stuetz, R.M. (2006) Characterization of Thiobacillus thioparus isolated from an activated sludge bioreactor used for hydrogen sulfide treatment, Journal of Applied Microbiology, Vol 101, pp. 1269–1281.
Beckingham, J.D. and Archibald, J.H. (1996). Field Guide to Ecosites of Northern Alberta, Canadian Forest Service Northwest Region, Northern Forestry Centre, Special Report #5.
Brady, N.C. and Weil, R.R. (1999) The Nature and Properties of Soils, 12th edition, 532 p.
Head, I.M., Saunders, J.R. and Pickup, R.W. (1998). Microbial Evolution, Diversity, and Ecology: A Decade of Ribosomal RNA Analysis of Uncultivated Microorganisms, Microbial Ecology. Vol. 35, pp. 1–31.
Holt, J.G., Krieg, N.R., Sneath, P.H.A., Staley, J.T. and Williams, S.T. (2000) Bergey’s Manual of Determinative Bacteriology, Ninth Edition, Lippincott Williams & Wilkins, Philadelphia, 787 p.
Johnson, E.A. and Miyanishi, K. (2008) Creating new landscapes and ecosystems: the Alberta Oil Sands, Annals of the New York Academy of Science, Vol. 1134, pp.120–145.
Kelly, D.P. and Wood, A.P. (2000) Reclassification of some species of Thiobacillus to the newly designated genera Acidithiobacillus gen. nov., Halothiobacillus gen. nov. and Thermithiobacillus gen. nov., International Journal of Systematic and Evolutionary Microbiology, Vol. 50, pp. 511–516.
Kirk, J.L., Beaudette, L.A, Hart, M., Moutoglis, P., Klironomos, J.N., Lee, H., and Trevors, J.T. (2004) Methods of studying soil microbial diversity. Journal of Microbiological Methods, Vol. 58, pp. 169–188.
Lane, D.J. (1991) 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds.). Nucleic Acid Techniques in Bacterial Systematics. Academic Press: Chichester, UK, pp. 115–175.
Mullis, K.B. and Faloona, F.A. (1987) Specific synthesis of DNA in vitro via a polymerase-catalyzed ain reaction, Methods Enzymology, Vol. 155, pp. 335–350.
Real, R. (1999). Tables of significant values of Jaccard's index of similarity. Miscelania Zoologica, Vol 22.1, pp. 29–40.
Real, R. and Vargar, J.M. (1996) The Probabilistic Basis of Jaccard's Index of Similarity. Systematic Biology, Vol. 45, pp. 380–385.
Salifu, K.F., Acton, C.A., Warner, C. and Anderson, B. (2009) Response of Forest Tree Seedlings and natural Vegetation to Fertilization: Suncor Dyke 5 Year Study, Canadian Land Reclamation Association, Issue 2.
Sanborn, P., Brockley, R., Mayer, B., Yun, M. and Prietzel, J. (2005). Sulphur Fertilizer of Lodgepole Pine: A Stable Isotype Tracer Study (E.P. 886.15) Establishment Report, BC Ministry of Forests, Technical Report #020.
Vidyalakshmi, R., Paranthaman, R. and Bhakyaraj, R. (2009). Sulphur Oxidizing Bacteria and Pulse Nutrition –Review. World Journal of Agricultural Sciences, Vol. 5 No. 3, pp. 270–278.
Wulf-Durand, P., Bryant, L.J. and Sly, L.I. (1997) PCR-Mediated Detection of Acidophilic, Bioleaching-Associated Bacteria, Applied and Environmental Microbiology, Vol. 63, No. 7, pp. 2944–2948.




© Copyright 2021, Australian Centre for Geomechanics (ACG), The University of Western Australia. All rights reserved.
Please direct any queries or error reports to repository-acg@uwa.edu.au