Fisher, NB 2012, 'Commercial microbial inocula – do they work?', in AB Fourie & M Tibbett (eds), Mine Closure 2012: Proceedings of the Seventh International Conference on Mine Closure
, Australian Centre for Geomechanics, Perth, pp. 449-457, https://doi.org/10.36487/ACG_rep/1208_39_Fisher
The important role that soil organisms play in nutrient cycling and plant health has long been recognised in the scientific literature, as well as in the agricultural and mining industries. The market for commercially available inocula of important nutrient cycling microbes aimed specifically at Australian native flora is not developed as agriculture with far fewer products.
Here the author presents the results of a trial utilising commercially available inocula marketed as capable of inoculating native flora. Eleven species of plants commonly found in the Box-Gum Woodlands of central western New South Wales and parts of Queensland were grown under nursery conditions and harvested after 150 days.
Faboideae species trialled were Acacia spectabilis (A. Cunn. ex Benth.), A. doratoxylon (A. Cunn.), Daviesia ulicifolia (Andrews), Pultenaea cinerascens (Maiden and Betche) and Hardenbergia violacea (Schneev.ex Stearn). Treatments included rhizobial, mycorrhizal + bacteria, and combined inoculation, with commercial nursery soil without inoculation as the control.
Preliminary results showed considerable variation between the species. A. spectabilis grew well regardless of treatment and formed root nodules with rhizobia (presumably) resident within the control nursery soil. H. violacea also nodulated when grown in the control treatment, but showed improved growth with both the rhizobial inoculation treatment with the combined treatment. A. doratoxylon, D. ulicifolia and P. cineracsens all showed increased survival and growth with each treatment; i.e. no survival in the control, low survival and poor growth after inoculation with mycorrhizal fungi + bacteria, increased growth again with rhizobial inoculation and markedly improved growth with combined inoculation.
In addition to D. viscosa, five eucalypt species were trialled; E. albens, E. conica, E. dawsonii, E. melliodora and E. moluccana. These plants were the subject of two treatments only, the control (as above) and inoculation with mycorrhizal fungi + bacteria. Inoculation actually suppressed growth in the majority of species a phenomenon often recorded in the literature.
Preliminary conclusions show that commercially available inocula can infect target plant species and may provide a useful addition to the tools available for rehabilitation in the absence of available topsoil with a viable microbial population.
Ardakani, M.R., Pietsch, G., Friedel, J.K., Schweiger, P., Maghaddam, A. and Raza, A. (2009) Effect of co-inoculation with rhizobia and mycorrhiza on root parameters of lucerne (Medicago sativa L.) under dry organic farming conditions. International Symposium of Root Research and Applications, RootRAP, 2–4 Sept 2009, Boku, Vienna, Austria.
Arriagada, C., Sampedro, I., Garcia-Romera, I. and Ocampo, J. (2009) Improvement of growth of Eucalyptus globulus and soil biological parameters with sewage sludge and inoculation with arbuscular mycorrhizal and saprobe fungi. Science of the Total Environment, 407, pp. 4799–4806.
Arumugam, R., Rajasekaran, S. and Nagarajan, S.M. (2010) Response of arbuscular mycorrhizal fungi and Rhizobium inoculation on growth and chlorophyll content of Vigna unguiculata Walp Va, Pusa 151. Journal of Applied Sciences and Environmental Management. 14(4), pp. 113–115.
Banning, N.C., Phillips, I.R., Jones, D.L. and Murphy, D.V. (2010) Development of Microbial Diversity and Functional Potential in Bauxite Residue Sand under Rehabilitation. Restoration Ecology. 19(101), pp. 78–87.
Bell, B., Wells, S., Jasper, D.A. and Abbott, L.K. (2003) Field inoculation with arbuscular mycorrhizal fungi in rehabilitation of mine sites with native vegetation, including Acacia spp. Australian Systematic Botany, 16, pp. 131–138.
Bisht, R., Chaturvedi, S., Srivastna, R., Sharma, A.K. and Jouri, B.N. (2009) Effect of arbuscualr mycorrhizal fungi, Pseudomonas flourescens and Rhizobium leguminosarum on the growth and nutrient stats of Dalbergia sissoo Roxb. Tropical Ecology 50 (2), pp. 231–242.
Brundrett, M. (1991) Mycorrhizas in Natural Ecosystems. In Advances in Ecological Research. Begon, M, Fitter, A. H. & Macfadyen, A. (Eds.) Vol. 21, Academic Press Limited, London, pp. 171-313.
Dell, B. (2002) Role of Mycorrhizal Fungi in Ecosystems, CMU Journal 1(1), pp. 47–60.
Fisher, N.B. (2011) Sustainable reintroduction of the nitrogen cycle post coal mining utilizing the legume-rhizobia symbiosis. Unpublished PhD Thesis, The University of Newcastle,
Gange, A., Gane, D.R.J., Chen, Y.L. and Gong, M.Q. (2005) Dual colonization of Eucalyptus urophylla ST Blake by arbuscular and ectomycorrhizal fungi affects levels of insect herbivore attack. Agricultural and Forest Entomology, 7 (3).
Geneva, M., Zehirov, G., Djonona, E., Kalayanova, N., Georgiev, G. and Stancheva, I. (2006) The effect of inoculation of pea plants with mycorrhizal fungi and Rhizobium on nitrogen and phosphorus assimilation. Plant Soil Environment, 52(10), pp. 435–440.
Groves, R. H. (1994) Australian Vegetation. 2nd edition, Cambridge University Press.
Jasper, D.A. (1994) Bioremediation of agricultural and forestry soils with symbiotic micro-organisms. Australian Journal of Soil Research, 32, pp. 1301–1319.
Lafay, B. and Burdon, J.J. (1998) Molecular diversity of rhizobia occurring on native shrubby legumes in south eastern Australia, Applied and Environmental Microbiology. Vol. 64(10), pp. 3989–3997.
Madejón, E., Dornmila, A.I., Madejón, P., Baker, A.J.M. and Woodrow, I.E. (2012) Biosolids, mycorrhizal fungi and Eucaltpts for phytostabilization purposes if sulphidic mine tailings. Agroforestry Systems. 84(3), pp. 389–399.
Marschner, H. and Bell, B. (1994) Nutrient uptake in mycorrhizal symbiosis. Plant and Soil. 159 (1), pp. 89–102.
Misbahzzaman, K. and Newton, A. (2006) Effect of dual arbuscular-ectomycorrhizal inocualtion on mycorrhizal formation and growth in E. camaldulensis Dehn. seedlings under different nutrient regimes. International Journal of Agriculture & Biology, 8(6), pp. 848–854.
Phillips, P.W.B. (2004) An economic assessment of the global inoculant industry. Online, Crop Management.
Richardson, D.M., Allsopp, N., D’Antonio, C.M., Milton, S.J. and Rejmánek, M. (2000) Plant invasions – the role of mutualisms. Biological Reviews, Vol. 75, pp. 65–93.
Slattery, J. and Pearce, D. (2002) Inoculants and Nitrogen Fixation in Vietnam (ed. D Herridge) ACAIR Proceedings 109e.
So, T., Ruthroff, K.X. and Dell, B. (2011) Seed and seedling responses to inoculation with mycorrhizal fungi and root nodule bacteria: implications for restoration of degraded Mediterranean-type Tuart woodlands. Ecological Management & Restoration, 12(2), pp. 157–160.
Thrall, P., Murray, B.R., Watkin, E.L.J., Woods, M.J., Baker, K., Burdon, J.J. and Brockwell, J. (2001) Bacterial partnerships enhance the value of native legumes in the rehabilitation of degraded agricultural lands. Ecological Management & Restoration: 2(3), pp. 233–235.