Authors: Guterres, J; Rossato, L; Pudmenzky, A; Doley, D; Whittaker, M; Schmidt, S


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Guterres, J, Rossato, L, Pudmenzky, A, Doley, D, Whittaker, M & Schmidt, S 2012, 'Micron-size metal-binding hydrogel particles improve germination and radicle elongation of Australian metallophyte grasses in mine waste rock and tailings', in AB Fourie & M Tibbett (eds), Mine Closure 2012: Proceedings of the Seventh International Conference on Mine Closure, Australian Centre for Geomechanics, Perth, pp. 517-531,

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Metal contamination of landscapes as a result of mining and other industrial activities is a pervasive problem worldwide. Metal contaminated soils often lack effective vegetation cover and are prone to contaminant leaching and dispersion through erosion, leading to contamination of the general environment. Hydrogels and similar hydrophilic and negatively-charged compounds are well-established ameliorants for wastes and tailings, but their application is constrained by transport (bulk), attributes, and cost. In this paper we demonstrate that metal-binding hydrogel particle amendments could be used to ameliorate substrates prior to planting, in order to enhance seedling emergence. In this study, micron-size thiol functional cross-linked acrylamide polymer hydrogel particles (X3) were synthesised and tested in laboratory-scale experiments on extremely saline and metal contaminated mine waste rock and tailings to determine: (i) their capacity to increase substrate water holding capacity (WHC); (ii) their metal-binding efficiency and capacity to reduce metal availability to plants to below the phytotoxicity threshold; and (iii) their effect on the germination characteristics and early radicle development of two Australian metallophyte grasses, Astrebla lappacea and Austrostipa scabra, under limiting and non-limiting water conditions. Addition of X3 to waste rock (18.4% dry weight) and tailings (3.2% dry weight) increased the WHC of both substrates, by more than 536% and 174% respectively, over the tested pH range from 2.0 to 6.3. X3 also significantly (P<0.05) lowered soluble concentrations of Al and Cu in leachate from amended waste rock, by up to 55 and 59% respectively between pH 3.2 and 6.3. Below pH 3.2, metal-binding efficiency declined to almost zero, suggesting a loss of particle functionality in extremely acidic conditions. X3 was not toxic to seed germination and significantly (P<0.05) increased germination percentages of A. lappacea and A. scabra in both waste rock and mine tailings in Petri dishes under controlled conditions. The highest germination percentages were recorded under a restricted water regime (substrates were watered to field capacity on the first day of the experiment but received no additional water thereafter). In A. lappacea, germination percentages increased to 43 and 10% in amended waste rock and tailings respectively compared to 9 and 0% in unamended treatments. In A. scabra, germination percentages increased from 0.5 to 24%, and from 6 to 21% in waste rock and tailings respectively. X3 also significantly (P<0.05) enhanced the radicle elongation of both species in contaminated waste rock and tailings. Under restricted water regime, radicle length of A. lappacea was increased to up to 19 and 13.5 mm in amended waste rock and tailings respectively as compared with 2 and 3 mm in unamended treatments. In A. scabra, radicle length was increased to up to 5.5 and 4.5 mm in amended waste rock and tailings respectively versus 0 and 1 mm in unamended treatments. Overall, greater radicle growth in X3 amended waste rock and tailings in water limited environments suggests that X3 was able to ameliorate metal toxicity to radicles, and provide moisture in water restricted conditions, which improved the imbibition and consequent germination of the seeds. X3 appears to have potential for the establishment of vegetation on contaminated land and wastes through a combination of reduced metal toxicity and increased soil-water availability. Together, these factors can potentially stabilise surfaces and reduce leaching or runoff of contaminants.

Abd El-Rehim, H.A., Hegazy, E.A. and Abd El-Mohdy, H.L. (2004) Radiation synthesis of hydrogels to enhance sandy soils water retention and increase plant performance, Journal of Apply Polymer Science, Vol. 93, pp. 1360–1371.
Abd El-Rehim, H.A., Hegazy, E.A. and Abd El-Mohdy, H.L. (2006) Effect of Various Environmental Conditions on the Swelling Property of PAAm/PAAcK Superabsorbent Hydrogel Prepared by Ionizing Radiation, Journal of Applied Polymer Science, Vol. 101, pp. 3955–3962.
Adriano, D.C. (2001) Trace elements in terrestrial environments: biogeochemistry, bioavailability, and risks of metals, USA: Springer, 867 p.
Agaba, H., Orikiriza, L.J.B., Obua, J., Kabasa, J.D., Worbes, M. and Huttermann, A. (2011) Hydrogel amendment to sandy soil reduces irrigation frequency and improves the biomass Agrostis stolonifera, Agricultural Sciences, Vol. 2, pp. 544–550.
Alvarez, E., Fernandez Marcos, M.L., Vaamonde, C. and Fernandez-Sanjurjo, M.J. (2003) Heavy metals in the dump of an abandoned mine in Galicia (NW Spain) and in the spontaneously occurring vegetation, Science of the Total Environment, Vol. 313, pp. 185–197.
Antonijevic, M.M., Dimitrijevic, M.D., Milic, S.M. and Nujkic, M.M. (2012) Metal concentrations in the soils and native plants surrounding the old flotation tailings pond of the Copper Mining and Smelting Complex Bor (Serbia), Journal of Environmental Monitoring, Vol. 14, pp. 866–877.
Bell, C.A., Smith, S.V., Whittaker, M.R., Whittaker, A.K., Gahan, L.R. and Monteiro, M.J. (2006) Surface-functionalized polymer nanoparticles for selective sequestering of heavy metals, Advanced Materials, Vol. 18, pp. 582–586.
Belsky, A.J., Amundson, R.G., Duxbury, J.M., Riha, S.J., Ali, A.R. and Mwonga, S.M. (1989) The effects of trees on their physical, chemical and biological environments in a semi-arid savanna in Kenya, The Journal of Applied Ecology, Vol. 26, pp. 1005‒1024.
Blodgett, A.M., Beattie, D.J., White, J.W. and Elliott, G.C. (1993) Hydrophilic polymers and wetting agents affect absorption and evaporative water loss, HortScience, Vol. 28, pp. 633–635.
Davies, F.T. Jr. and Castro-Jimenez, Y. (1989) Water relations of Lagerstroemia indica grown in amended media under drought stress, Scientia Horticulturae, Vol. 41, pp. 97–104.
de Varennes, A. and Queda, C. (2005) Application of an insoluble polyacrylate polymer to copper-contaminated soil enhances plant growth and soil quality, Soil Use and Management, Vol. 21, pp. 410–414.
de Varennes, A., Qu, G., Cordovil, C. and Goncalves, P. (2011) Soil quality indicators response to application of hydrophilic polymers to a soil from a sulfide mine, Journal of Hazardous Materials, Vol. 192, pp. 1836–1841.
Dorraji, S.S., Golchin, A. and Ahmadi, S. (2010) The Effects of Hydrophilic Polymer and Soil Salinity on Corn Growth in Sandy and Loamy Soils, Clean-Soil Air Water, Vol. 38, pp. 584–591.
Ellis, R.H. and Roberts, E.H. (1981) The qualification of ageing and survival in orthodox seeds, Seed Science and Technology, Vol. 9, pp. 373–409.
El-Temsah, Y.S. and Joner, E.J. (2012) Impact of Fe and Ag nanoparticles on seed germination and differences in bioavailability during exposure in aqueous suspension and soil, Environmental Toxicology, Vol. 27, pp. 42–49.
Falatah, A.M., Choudhary, M.I. and Al-Omran, A.M. (1996) Changes in some chemical properties of arid soils as affected by synthetic polymers, Arid Soil Research and Rehabilitation, Vol. 10, pp. 277–285.
Guiwei, Q., de Varennes, A. and Cunha-Queda, C. (2008) Remediation of a mine soil with insoluble polyacrylate polymers enhances soil quality and plant growth, Soil Use and Management, Vol. 24, pp. 350–356.
Hegazy, A.K., Abdel-Ghani, N.T. and El-Chaghaby, G.A. (2011) Phytoremediation of industrial wastewater potentiality by Typha domingensis, International Journal of Environmental Science and Technology, Vol. 8, pp. 639–648.
Hussien, R.A., Donia, A.M., Atia, A.A., El-Sedfy, O.F., Abd El-Hamid, A.R. and Rashad, R.T. (2012) Studying some hydro-physical properties of two soils amended with kaolinite-modified cross-linked poly-acrylamides, Catena, Vol. 92, pp. 172–178.
Huttermann, A., Zommorodi, M. and Reisa, K. (1999) Addition of hydrogels to soil for prolonging the survival of Pinus halepensis seedlings subjected to drought, Soil and Tillage Research, Vol. 50, pp. 295–304.
Keeling, S.M. and Werren, G. (2005) Phytoremediation: The uptake of metals and metalloids by Rhodes grass grown on metal-contaminated soil, Remediation Journal, Vol. 15, pp. 53–61.
Kikui, S., Sasaki, T., Maekawa, M., Miyao, A., Hirochika, H., Matsumoto, H. and Yamamoto, Y. (2005) Physiological and genetic analyses of aluminium tolerance in rice, focusing on root growth during germination, Journal of Inorganic Biochemistry, Vol. 99, pp. 1837–1844.
Kopittke, P.M., Blamey, F.P.C., Asher, C.J. and Menzies, N.W. (2010) Trace metal phytotoxicity in solution culture: a review, Journal of Experimental Botany, Vol. 61, pp. 945–954.
Ma, Y.H., He, X., Zhang, P., Zhang, Z.Y., Guo, Z., Tai, R.Z., Xu, Z.J., Zhang, L.J., Ding, Y.Y., Zhao, Y.L. and Chai, Z.F. (2011) Phytotoxicity and biotransformation of La2O3 nanoparticles in a terrestrial plant cucumber (Cucumis sativus), Nanotoxicology, Vol. 5, pp. 743–753.
Meda, A.R. and Furlani, P.R. (2005) Tolerance to aluminium toxicity by tropical leguminous plants used as cover crops, Brazilian Archives of Biology and Technology, Vol. 48, pp. 309–317.
Mendez, M.O. and Maier, R.M. (2008) Phytostabilization of mine tailings in arid and semiarid environments – an emerging remediation technology, Environmental Health Perspectives, Vol. 116, pp. 278–283.
Menzies, N.W., Donn, M.J. and Kopittke, P.M. (2007) Evaluation of extractants for estimation of phytoavailable trace metals in soils, Environmental Pollution, Vol. 145, pp. 121–130.
Mulvey, P.J. and Elliott, G.L. (2000) Toxicities in soils, Soils: their properties and management, 2nd edition, P.E.V. Charman and B.W. Murphy (eds), Oxford University Press, South Melbourne, Australia, p. 254.
Mushtaq, Y.K. (2011) Effect of nanoscale Fe3O4, TiO2 and carbon particles on cucumber seed germination, Journal of Environmental Science and Health, Part A, Vol. 46, pp. 1732–1735.
Nair, R., Poulose, A.C., Nagaoka, Y., Yoshida, Y., Maekawa, T. and Kumar, D.S. (2011) Uptake of FITC labeled silica nanoparticles and quantum dots by rice seedlings: Effects on seed germination and their potential as biolabels for plants, Journal of Fluorescence, Vol. 21, pp. 2057–2068.
Nedunuri, K.V., Lowell, C., Meade, W., Vonderheide, A.P. and Shann, J.R. (2010) Management Practices and Phytoremediation by Native Grasses, International Journal of Phytoremediation, Vol. 12, pp. 200–214.
Qu, G. and de Varennes, A. (2010) Use of hydrophilic polymers from diapers to aid the establishment of Spergularia purpurea in a mine soil, Journal of Hazardous Materials, Vol. 178, pp. 956–962.
Rayment, G.E. and Higginson, F.R. (1992) Australian laboratory handbook of soil and water chemical methods, Melbourne/Sydney, Inkata Press.
Rossato, L., MacFarlane, J., Whittaker, M., Pudmenzky, A., Doley, D., Schmidt, S. and Monteiro, M.J. (2011) Metal-binding particles alleviate lead and zinc toxicity during seed germination of metallophyte grass Astrebla lappacea, Journal of Hazardous Materials, Vol. 190, pp. 772–779.
Rossato, L., Pudmenzky, A., Doley, D., Monteiro, M.J., Whittaker, M.R., Schmidt, S., MacFarlane, J. and Baker, A.J.M. (2009) Metal-binding particles enhance germination and radicle tolerance index of the metallophyte grass Astrebla lappacea under phytotoxic lead and zinc conditions, in Proceedings Fourth International Conference on Mine Closure (Mine Closure 2009), A.B. Fourie and M. Tibbett (eds), 9‒11 September 2009, Perth, Australia, Australian Centre for Geomechanics, Perth, pp. 302–310.
Salt, D.E., Blaylock, M., Kumar, N.P.B.A., Dushenkov, V., Ensley, B.D., Chet, I. and Raskin, I. (1995) Phytoremediation: a novel strategy for the removal of toxic metals from the environment using plants, Biotechnology, Vol. 13, pp. 468–474.
Shanker, A.K., Cervantes, C., Loza-Tavera, H. and Avudainayagam, S. (2005) Chromium toxicity in plants, Environment International, Vol. 31, pp. 739–753.
Singh, R., Misra, V. and Singh, R.P. (2012) Removal of Cr(VI) by Nanoscale Zero-valent Iron (nZVI) from Soil Contaminated with Tannery Wastes, Bulletin of Environmental Contamination and Toxicology, Vol. 88, pp. 210–214.
Sun, Y.P., Li, X., Cao, J., Zhang, W. and Wang, H.P. (2006) Characterization of zero-valent iron nanoparticles, Advances in Colloid and Interface Science, Vol. 120, pp. 47–56.
The State of Queensland (2011) Salinity Management Handbook, 2nd edition, Department of Environment and Resource Management, Brisbane, Australia.
Tiedemann, A.R. and Klemmedson, J.O. (2004) Responses of desert grassland vegetation to mesquite removal and regrowth, Journal of Range Management, Vol. 57, pp. 455–465.
Torres, M.O. and de Varennes, A. (1998) Remediation of a sandy soil artificially contaminated with copper using a polyacrylate polymer, Soil Use and Management, Vol. 14, pp. 106–110.
USEPA (2005) United States Environmental Protection Agency. Nanotechnology for site remediation, in US EPA Workshop on nanotechnology for site remediation, October 20–21, 2005, US Department of Commerce, Washington DC.
Williams, D.J. and Currey, N.A. (2002) Engineering closure of an open pit gold operation in a semi-arid climate, International Journal of Surface Mining, Reclamation and Environment, Vol. 16, pp. 270–288.

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